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SARS-COV-2 Spike RBD ( N501Y,K417N,E484K ) Protein

Product Info

Recombinant SARS-COV-2 Spike RBD ( N501Y,K417N,E484K ) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(N501Y,K417N,E484K).[Accession | QHD43416.1]

Molecular Weight

The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.


Less than 1EU per μg by the LAL method.


> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC


Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.


Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.


The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

SARS-COV-2 Spike RBD ( N501Y,K417N,E484K ) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.


The purity of SARS-COV-2 Spike RBD ( N501Y,K417N,E484K ) is greater than 95% as determined by SEC-HPLC.


Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K), His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA.


The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.


S protein RBD; S glycoprotein RBD; Spike protein RBD


(1)Larry J Anderson, Ralph S Baric. Emerging Human Coronaviruses - Disease Potential and Preparedness[J]. N Engl J Med, 2012, 367(19):1850-1852.

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