Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44DB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met22-Arg734

Catalog: SEM-HM44DB

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Product Description Recombinant Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734.

Product Background

Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

Product Category

Recombinant Protein

Target

Semaphorin 4D/SEMA4D/CD100
Synonyms Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4

Gene/Protein ID

Q92854-1

Species

Human

Exact Sequence

Met22-Arg734
Molecular Weight The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
The purity of Biotinylated Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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