Human SIRP Gamma/CD172g Protein (SRP-HM40G)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SIRP Gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu29-Pro360

Catalog: SRP-HM40G

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Product Description	Recombinant Human SIRP Gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360.
Product Background	Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.
Product Category	Recombinant Protein

Target	SIRP Gamma/CD172g
Synonyms	CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1
Gene/Protein ID	Q9P1W8
Species	Human
Exact Sequence	Glu29-Pro360
Molecular Weight	The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human SIRP Gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human SIRP Gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46ug/ml determined by ELISA.
	The purity of Human SIRP Gamma is greater than 95% as determined by SEC-HPLC.
	Human SIRP Gamma on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%..