• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >90000 pmol/min/ug.
  • Amino Acid Range: Lys33-Asp386
Catalog #ACP-HM101
Price
Regular price $390.00
Regular price Sale price $390.00
/
Size
Avantor VWR Fisher Scientific

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Human Prostatic Acid Phosphatase Protein (ACP-HM101) Summary

Product Description

 Recombinant Human Prostatic Acid Phosphatase Protein is expressed from HEK293 with His tag at the C-terminus.It contains Lys33-Asp386.

Product Background

 Human PAcP is classically known as a prostate epithelium-specific 100 kDa glycoprotein [20], consisting of two subunits with molecular weight of about 50 kDa each [21]. In normal differentiated prostate epithelia, PAcP protein can be detected intracellularly as the cellular form (cPAcP) and in seminal fluid as the secretory form (sPAcP). The two forms of PAcP protein are apparently transcribed from the same gene followed by different post-transcriptional modifications. Circulating PAcP activity is serving as a surrogate marker for diagnosing PCa as well as for evaluating the efficacy of ADT for advanced PCa.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Prostatic Acid Phosphatase Protein (ACP-HM101) Specifications/Details

Protein
 Prostatic Acid Phosphatase
Synonyms
 PAP; 5'-NT; ACPP; ACP-3; TMPase; TM-PAP
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys33-Asp386
Molecular Weight
 The protein has a predicted MW of 42.61 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >90000 pmol/min/ug.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM NaAc, 20% Glycerol (pH 5.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Prostatic Acid Phosphatase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Prostatic Acid Phosphatase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Prostatic Acid Phosphatase is greater than 95% as determined by SEC-HPLC. The purity of Human Prostatic Acid Phosphatase is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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