Mouse AREG Protein, Ultra Low Endotoxin (AEG-MM201-UL)
  • Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Val100-Ala248
Catalog #AEG-MM201-UL
Regular price $363.00
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Mouse AREG Protein, Ultra Low Endotoxin (AEG-MM201-UL) Summary

Product Description

Recombinant Mouse AREG Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Val100-Ala248.

Product Background

Amphiregulin (AREG) is a ligand of the epidermal growth factor receptor (EGFR), a widely expressed transmembrane tyrosine kinase. AREG is synthesized as a membrane-anchored precursor protein that can engage in juxtacrine signaling on adjacent cells. Alternatively, after proteolytic processing by cell membrane proteases, mainly TACE/ADAM17, AREG is secreted and behaves as an autocrine or paracrine factor.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse AREG Protein, Ultra Low Endotoxin (AEG-MM201-UL) Specifications/Details

Protein
AREG
Synonyms
Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Val100-Ala248
Molecular Weight
The protein has a predicted MW of 38.6 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Product Tag
N-hFc (IgG1)
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse AREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse AREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse AREG is greater than 95% as determined by SEC-HPLC. The purity of Mouse AREG is greater than 95% as determined by SEC-HPLC.
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