Product Description |
Recombinant Mouse ASPH Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ile324-Ile741. |
Product Background |
Aspartate β-hydroxylase (ASPH) is silent in normal adult tissues only to re-emerge during oncogenesis where its function is required for generation and maintenance of malignant phenotypes. Exosomes enable prooncogenic secretome delivering and trafficking for long-distance cell-to-cell communication.Expression profiling of Notch signaling components positively correlates with ASPH expression in breast cancer patients, confirming that ASPH-Notch axis acts functionally in breast tumorigenesis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
ASPH |
Synonyms |
Aspartate beta-hydroxylase; ASP beta-hydroxylase; Asph; Bah;Aspartate β-hydroxylase; ASP β-hydroxylase |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ile324-Ile741 |
Molecular Weight |
The protein has a predicted MW of 49.4 kDa same as Bis-Tris PAGE result. |
Product Tag |
N-His |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Mouse ASPH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse ASPH is greater than 95% as determined by SEC-HPLC. |
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