Biotinylated Cynomolgus PSMA/FOLH1 Protein (Primary Amine Labeling) (PSM-CM110B)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Anti-PSMA Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Cynomolgus PSMA, His Tag with the EC50 of 21.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys44-Ala750

Catalog: PSM-CM110B

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Product Description Recombinant Biotinylated Cynomolgus PSMA/FOLH1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys44-Ala750.

Product Background

 Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

Product Category

 Recombinant Protein

Target

 PSMA/FOLH1

Gene/Protein ID

 A0A2K5VNZ0

Species

 Cynomolgus

Exact Sequence

 Lys44-Ala750
Molecular Weight The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Anti-PSMA Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Cynomolgus PSMA, His Tag with the EC50 of 21.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-PSMA Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Cynomolgus PSMA, His Tag with the EC50 of 21.2ng/ml determined by ELISA.
Biotinylated Cynomolgus PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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