Biotinylated Human B7-H6 Protein (BH7-HM476B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: The affinity constant of 0.227 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Asp25-Ser262

Catalog: BH7-HM476B

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Product Description	Recombinant Biotinylated Human B7-H6 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Ser262.
Product Background	B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
Product Category	Recombinant Protein

Target	B7-H6
Synonyms	B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6
Gene/Protein ID	Q68D85
Species	Human
Exact Sequence	Asp25-Ser262
Molecular Weight	The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.227 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.227 uM as determined in SPR assay (Biacore T200).
	The purity of Biotinylated Human B7-H6 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human B7-H6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.