Biotinylated Human CA125/MUC16 Protein (MUC-HM426B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human MSLN, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 12.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp12783-Ser13467

Catalog: MUC-HM426B

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Product Description Recombinant Biotinylated Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp12783-Ser13467.

Product Background

 MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.

Product Category

 Recombinant Protein

Target

 CA125/MUC16

Gene/Protein ID

 Q8WXI7

Species

 Human

Exact Sequence

 Asp12783-Ser13467
Molecular Weight The protein has a predicted MW of 77.98 kDa. Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human MSLN, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 12.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MSLN, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 12.4ng/ml determined by ELISA.
Biotinylated Human CA125 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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