Biotinylated Human CD161 Protein (CD1-HM461B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Anti-CD161 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD161, His Tag with the EC50 of 32.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln67-Ser225

Catalog: CD1-HM461B

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Product Description	Recombinant Biotinylated Human CD161 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln67-Ser225.
Product Background	CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.
Product Category	Recombinant Protein

Target	CD161
Synonyms	KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
Gene/Protein ID	Q12918-1
Species	Human
Exact Sequence	Gln67-Ser225
Molecular Weight	The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 41-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Anti-CD161 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD161, His Tag with the EC50 of 32.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-CD161 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD161, His Tag with the EC50 of 32.5ng/ml determined by ELISA.
	The purity of Biotinylated Human CD161 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.