Biotinylated Human CD20/MS4A1 Protein (CD2-HE120B)

  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD20, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile141-Ser188

Catalog: CD2-HE120B

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Product Description Recombinant Biotinylated Human CD20/MS4A1 Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus.It contains Ile141-Ser188.

Product Background

 B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

Product Category

 Recombinant Protein

Target

 CD20/MS4A1
Synonyms MS4A1;CD20;MS4A-1;B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 11

Gene/Protein ID

 P11836-1

Species

 Human

Exact Sequence

 Ile141-Ser188
Molecular Weight The protein has a predicted MW of 16.8 kDa same as Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human CD20, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in 20mM PB, 10% Glycerol (pH 7.0).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CD20, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA.
Biotinylated Human CD20 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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