Biotinylated Human CD24 Protein (Primary Amine Labeling) (CD2-HM324B)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser27-Gly59

Catalog: CD2-HM324B

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Product Description Recombinant Biotinylated Human CD24 Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ser27-Gly59.

Product Background

 CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.

Product Category

 Recombinant Protein

Target

 CD24
Synonyms CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043

Gene/Protein ID

 P25063-1

Species

 Human

Exact Sequence

 Ser27-Gly59
Molecular Weight The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-mFc

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31ug/ml determined by ELISA.
The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD24 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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