Biotinylated Human CD93/C1q R1 Protein (CD9-HM493B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr22-Lys580

Catalog: CD9-HM493B

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Product Description Recombinant Biotinylated Human CD93/C1q R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr22-Lys580.

Product Background

 CD93 has been shown critical roles in inflammatory and immune diseases. CD93 silencing increased IL-6 and TSLP, but not IL-33 levels in culture supernatants. HDM-induced asthma mice showed significant airway hyperresponsiveness and inflammation with Th2 cytokine activation, along with decreased CD93 expression in bronchial epithelial cells and lung homogenates but increased serum CD93 levels.

Product Category

 Recombinant Protein

Target

 CD93/C1q R1
Synonyms C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; CD93; C1QR1; MXRA4; ECSM3; dJ737E23.1

Gene/Protein ID

 Q9NPY3

Species

 Human

Exact Sequence

 Thr22-Lys580
Molecular Weight The protein has a predicted MW of 61.2 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human CD93, His-Avi Tag immobilized on CM5 Chip can bind Human IGFBP-7, His Tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA (QC Test).
The purity of Biotinylated Human CD93 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD93 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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