Biotinylated Human CEACAM-8/CD66b Protein (CAM-HM408B)
Catalog: CAM-HM408B
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| Product Description | Recombinant Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln35-Ser319. |
Product Background |
Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes. |
Product Category |
Recombinant Protein |
Target |
CEACAM-8/CD66b |
Gene/Protein ID |
NP_001807.2 |
Species |
Human |
Exact Sequence |
Gln35-Ser319 |
| Molecular Weight | The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 6.13 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human CEACAM-8 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human CEACAM-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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