Biotinylated Human CTGF/CCN2 Protein (CGF-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CTGF, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln27-Ala349

Catalog: CGF-HM401B

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Product Description Recombinant Biotinylated Human CTGF/CCN2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln27-Ala349.

Product Background

 Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.

Product Category

 Recombinant Protein

Target

 CTGF/CCN2
Synonyms IGFBP8; IBP-8; CCN2; NOV2; HCS24; CTGF; CTGRP; Fisp12; MGC102839

Gene/Protein ID

 Q5M8T4-1

Species

 Human

Exact Sequence

 Gln27-Ala349
Molecular Weight The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human CTGF, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CTGF, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Biotinylated Human CTGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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