Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB)

  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met178-His266

Catalog: DKK-HM51CB

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Product Description Recombinant Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met178-His266.

Product Background

 Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

Product Category

 Recombinant Protein

Target

 DKK1 C terminal Domain

Gene/Protein ID

 O94907

Species

 Human

Exact Sequence

 Met178-His266
Molecular Weight The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-hFc-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA.
Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test).
Biotinylated Human DKK1 C terminal Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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