Biotinylated Human DKK1 Protein (DKK-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Human DKK1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Thr32-His266

Catalog: DKK-HM401B

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Product Description	Recombinant Biotinylated Human DKK1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr32-His266.
Product Background	Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Product Category	Recombinant Protein

Target	DKK1
Gene/Protein ID	O94907
Species	Human
Exact Sequence	Thr32-His266
Molecular Weight	The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human DKK1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human DKK1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA.
	Immobilized Anti-DKK1 Antibody, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
	The purity of Biotinylated Human DKK1 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.