Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)
Catalog: CAM-HM4EPB
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| Product Description | Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Lys265. |
Product Background |
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
Product Category |
Recombinant Protein |
Target |
EpCAM/TROP1 |
| Synonyms | CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; 17-1A; 323/A3; ACSTD1; DIAR5; GA733-2; gp40; HNPCC8; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; ESA; KS1,4; KSA; MIC18; MK1 |
Gene/Protein ID |
P16422 |
Species |
Human |
Exact Sequence |
Gln24-Lys265 |
| Molecular Weight | The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 14.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 14.4ng/ml determined by ELISA. |
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The purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.. |
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