Biotinylated Human Fc gamma RI/CD64 Protein (FRI-HM464B)
Catalog: FRI-HM464B
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| Product Description | Recombinant Biotinylated Human Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln16-Pro288. |
Product Background |
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma- RI (also known as CD64), Fc gamma- RII (CD32), and Fc gamma- RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. |
Product Category |
Recombinant Protein |
Target |
Fc gamma RI/CD64 |
| Synonyms | CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI |
Gene/Protein ID |
P12314-1 |
Species |
Human |
Exact Sequence |
Gln16-Pro288 |
| Molecular Weight | The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human Fc gamma RI is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Fc gamma RI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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