Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-FcRH5 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln16-Gly851

Catalog: FCR-HM101B

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Product Description Recombinant Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln16-Gly851.

Product Background

 FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.

Product Category

 Recombinant Protein

Target

 FcRH5/FcRL5

Gene/Protein ID

 AAK93971

Species

 Human

Exact Sequence

 Gln16-Gly851
Molecular Weight The protein has a predicted MW of 92.38 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Anti-FcRH5 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-FcRH5 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 46.0ng/ml determined by ELISA.
The purity of Biotinylated Human FcRH5 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human FcRH5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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