Biotinylated Human FcRn Protein (FRN-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Exact Sequence: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog: FRN-HM401B

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Product Description Recombinant Biotinylated Human FcRn Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala24-Ser297(FCGRT)&Ile21-Met119(B2M).

Product Background

 The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Product Category

 Recombinant Protein

Target

 FcRn
Synonyms FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M

Gene/Protein ID

 P55899(FCGRT)&P61769((B2M)

Species

 Human

Exact Sequence

 Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 0.557 uM asdetermined in SPR assay (Biacore T200).
The purity of Biotinylated Human FcRn is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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