Biotinylated Human FGFR1 beta (IIIc) Protein (FGF-HM41CB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Anti-FGFR1 Anitibody at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human FGFR1 beta (IIIc) , His Tag with the EC50 of 0.42ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys158-Thr355

Catalog: FGF-HM41CB

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Product Description Recombinant Biotinylated Human FGFR1 beta (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys158-Thr355.

Product Background

 Fibroblast growth factor receptor 1 (FGFR1) transmits signals through the plasma membrane regulating essential cellular processes like division, motility, metabolism, and death. Overexpression of FGFR1 is observed in numerous tumors and thus constitutes an attractive molecular target for selective cancer treatment.

Product Category

 Recombinant Protein

Target

 FGFR1 beta (IIIc)

Gene/Protein ID

 P11362-7

Species

 Human

Exact Sequence

 Lys158-Thr355
Molecular Weight The protein has a predicted MW of 25.23 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Anti-FGFR1 Anitibody at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human FGFR1 beta (IIIc) , His Tag with the EC50 of 0.42ug/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-FGFR1 Anitibody at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human FGFR1 beta (IIIc) , His Tag with the EC50 of 0.42ug/ml determined by ELISA.
Biotinylated Human FGFR1 beta (IIIc) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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