Biotinylated Human FGL1 Protein (Primary Amine Labeling) (FGL-HM211B)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp64-Asn305

Catalog: FGL-HM211B

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Product Description Recombinant Biotinylated Human FGL1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp64-Asn305.

Product Background

 Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.

Product Category

 Recombinant Protein

Target

 FGL1

Gene/Protein ID

 Q08830

Species

 Human

Exact Sequence

 Asp64-Asn305
Molecular Weight The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-hFc

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
The purity of Biotinylated Human FGL1 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human FGL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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