Biotinylated Human GDF15 Protein (GDF-HE415B)

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Biotinylated: yes
Activity: Immobilized Human GFRAL, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 24ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala197-Ile308

Catalog: GDF-HE415B

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Product Description	Recombinant Biotinylated Human GDF15 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala197-Ile308.
Product Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Product Category	Recombinant Protein

Target	Biotinylated GDF15
Synonyms	GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Gene/Protein ID	Q99988-1
Species	Human
Exact Sequence	Ala197-Ile308
Molecular Weight	The protein has a predicted MW of 15.19 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human GFRAL, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 24ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GFRAL, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 24ng/ml determined by ELISA.
	Biotinylated Human GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.