Biotinylated Human GPRC5D Protein-VLP (GPR-HM05PB)

  • Express System: HEK293
  • Purity: > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/well) on the plate. Add serial dilutions of Anti-CD3×GPRC5D Bispecific Antibody, hFc Tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5ng/ml determined by ELISA. The affinity constant of 0.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met1-Val345

Catalog: GPR-HM05PB

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Product Description Recombinant Biotinylated Human GPRC5D Protein-VLP is expressed from HEK293.It contains Met1-Val345.

Product Background

 Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.

Product Category

 Recombinant Protein

Target

 GPRC5D
Synonyms GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor

Gene/Protein ID

 Q9NZD1-1

Species

 Human

Exact Sequence

 Met1-Val345
Molecular Weight The target protein has a predicted MW of 39.6 kDa.

Activity

 Immobilized Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/well) on the plate. Add serial dilutions of Anti-CD3×GPRC5D Bispecific Antibody, hFc Tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5ng/ml determined by ELISA. The affinity constant of 0.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Express System

 HEK293
Purity > 95% as determined by HPLC
Endotoxin Less than 1EU per ul by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in PBS, 300mM L-Arginine (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human GPRC5D VLP captured on SA Chip can bind Anti-GPRC5D antibody, hFc Tag with an affinity constant of 0.30 nM as determined in SPR assay (Biacore T200) (QC Test).
Immobilized Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/well) on the plate. Add serial dilutions of Anti-CD3×GPRC5D Bispecific Antibody, hFc Tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5ng/ml determined by ELISA.
The purity of Biotinylated Human GPRC5D VLP is greater than 95% as determined by SEC-HPLC.

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