Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CTB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Anti-HLA-G Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with the EC50 of 19.9ng/ml determined by ELISA (QC Test). The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide

Catalog: HLG-HM41CTB

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Product Description Recombinant Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.

Product Background

 HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

Product Category

 Recombinant Protein

Target

 HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer
Synonyms HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
MHC Form Tetramer
Allele HLA-G

Gene/Protein ID

 P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL

Species

 Human

Exact Sequence

 Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Activity

 Immobilized Anti-HLA-G Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with the EC50 of 19.9ng/ml determined by ELISA (QC Test). The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His-Avi Tag with an affinity constant of 4.38 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-HLA-G Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with the EC50 of 19.9ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-LILRB2 Antibody were added into Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag: Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.29ug/ml.
Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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