Biotinylated Human IL-10 R alpha Protein (IL1-HM4RAB)
Catalog: IL1-HM4RAB
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| Product Description | Recombinant Biotinylated Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His22-Asn235. |
Product Background |
The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD. |
Product Category |
Recombinant Protein |
Target |
IL-10R alpha |
Gene/Protein ID |
Q13651 |
Species |
Human |
Exact Sequence |
His22-Asn235 |
| Molecular Weight | The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 42-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.0ng/ml determined by ELISA. |
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The purity of Biotinylated Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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