Biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein (ILB-HM4ABB)
Catalog: ILB-HM4ABB
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| Product Description | Recombinant Biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg20-Pro189 (Human IL-23 alpha) and Met23-Ser335 (Mouse IL-12 beta). |
Product Background |
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor. |
Product Category |
Recombinant Protein |
Target |
IL-23 alpha&IL-12 beta |
Gene/Protein ID |
Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta) |
Species |
Human&Mouse |
Exact Sequence |
Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta) |
| Molecular Weight | The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22-25 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human IL-23 alpha & Mouse IL-12 beta, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 28.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human IL-23 alpha & Mouse IL-12 beta, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 28.1ng/ml determined by ELISA. |
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The purity of Biotinylated Human IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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