Biotinylated Human LAP (TGF beta 1) Protein (LAP-HM4B1B)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu30-Arg278(C33S)

Catalog: LAP-HM4B1B

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Product Description Recombinant Biotinylated Human LAP (TGF beta 1) Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu30-Arg278(C33S).

Product Background

 Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.

Product Category

 Recombinant Protein

Target

 LAP (TGF beta 1)
Synonyms CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1

Gene/Protein ID

 P01137

Species

 Human

Exact Sequence

 Leu30-Arg278(C33S)
Molecular Weight The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86ug/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86ug/ml determined by ELISA.
Biotinylated Human LAP (TGF beta 1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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