Biotinylated Human LDLR Protein (LDL-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala22-Arg788

Catalog: LDL-HM401B

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Product Description	Recombinant Biotinylated Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala22-Arg788.
Product Background	The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.
Product Category	Recombinant Protein

Target	LDLR
Synonyms	LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH
Gene/Protein ID	P01130-1
Species	Human
Exact Sequence	Ala22-Arg788
Molecular Weight	The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Biotinylated Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
	The purity of Biotinylated Human LDLR is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human LDLR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.