Biotinylated Human Peptide Ready HLA-E*01:03&B2M Monomer-Protein (MHC-HM42RB)
Catalog: MHC-HM42RB
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| Product Description | Recombinant Biotinylated Human Peptide Ready HLA-E*01:03&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Ile305(HLA-E*01:03) and Ile21-Met119(B2M). |
Product Background |
HLA-E*01:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-E*01:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
Product Category |
Recombinant Protein |
Target |
Peptide Ready HLA-E*01:03&B2M Monomer |
| MHC Form | Monomer |
| Allele | HLA-E*01:03 |
Gene/Protein ID |
P13747(HLA-E*01:03)&P61769(B2M) |
Species |
Human |
Exact Sequence |
Gly25-Ile305(HLA-E*01:03) & Ile21-Met119(B2M) |
| Molecular Weight | The protein has a predicted MW of 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Biotinylated Human Peptide Ready HLA-E*01:03&B2M Monomer is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Peptide Ready HLA-E*01:03&B2M Monomer-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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