Biotinylated Human Siglec-4a/MAG Protein (SIG-HM44AB)
Catalog: SIG-HM44AB
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| Product Description | Recombinant Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly20-Pro516. |
Product Background |
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). |
Product Category |
Recombinant Protein |
Target |
Siglec-4a/MAG |
| Synonyms | MAG; Siglec4a; Siglec-4a; S-MAG; GMA |
Gene/Protein ID |
P20916 |
Species |
Human |
Exact Sequence |
Gly20-Pro516 |
| Molecular Weight | The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-Siglec-4a Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 24.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Siglec-4a Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 24.4ng/ml determined by ELISA. |
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The purity of Biotinylated Human Siglec-4a is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Siglec-4a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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