Biotinylated Human Tim-3/HAVCR2 Protein (TIM-HM431B)
Catalog: TIM-HM431B
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| Product Description | Recombinant Biotinylated Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Arg200. |
Product Background |
Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. |
Product Category |
Recombinant Protein |
Target |
Tim-3/HAVCR2 |
| Synonyms | CD366; TIM3; TIM-3; TIMD-3; TIM 3;-FLJ14428; HAVCR2; KIM3 |
Gene/Protein ID |
Q8TDQ0-1 |
Species |
Human |
Exact Sequence |
Ser22-Arg200 |
| Molecular Weight | The protein has a predicted MW of 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-Tim-3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Tim-3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 13.2ng/ml determined by ELISA. |
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The purity of Biotinylated Human Tim-3 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Tim-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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