Biotinylated Human TNFSF15 Protein (Primary Amine Labeling) (FSF-HM115B)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human TNFSF15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu72-Leu251

Catalog: FSF-HM115B

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Product Description Recombinant Biotinylated Human TNFSF15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-terminus.It contains Leu72-Leu251.

Product Background

 TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Product Category

 Recombinant Protein

Target

 TNFSF15

Gene/Protein ID

 O95150-1

Species

 Human

Exact Sequence

 Leu72-Leu251
Molecular Weight The protein has a predicted MW of 21.57 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human TNFSF15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human TNFSF15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Immobilized Mouse DR3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 0.69ug/ml determined by ELISA (QC Test).
The purity of Biotinylated Human TNFSF15 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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