Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) (FSF-HM416B)
Catalog: FSF-HM416B
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| Product Description | Recombinant Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Asp91-Leu251. |
Product Background |
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
Product Category |
Recombinant Protein |
Target |
TNFSF15 trimer |
Gene/Protein ID |
O95150-1 |
Species |
Human |
Exact Sequence |
Asp91-Leu251 |
| Molecular Weight | The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Mouse DR3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 (Trimer), His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Mouse DR3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 (Trimer), His Tag with the EC50 of 0.16ug/ml determined by ELISA. |
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The purity of Biotinylated Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human TNFSF15 Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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