Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)
Catalog: TFR-HM401B
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| Product Description | Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760. |
Product Background |
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
Product Category |
Recombinant Protein |
Target |
Transferrin R/CD71 |
Gene/Protein ID |
P02786 |
Species |
Human |
Exact Sequence |
Cys89-Phe760 |
| Molecular Weight | The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 80-85 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-Transferrin R Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 41.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Transferrin R Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 41.4ng/ml determined by ELISA. |
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The purity of Biotinylated Human Transferrin R is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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