Biotinylated Human TROP-2/TACSTD2 Protein (TRP-HM421B)
Catalog: TRP-HM421B
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| Product Description | Recombinant Biotinylated Human TROP-2/TACSTD2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His27-Thr274. |
Product Background |
Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. |
Product Category |
Recombinant Protein |
Target |
TROP-2/TACSTD2 |
| Synonyms | EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2 |
Gene/Protein ID |
P09758 |
Species |
Human |
Exact Sequence |
His27-Thr274 |
| Molecular Weight | The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 46-55 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human TROP-2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human TROP-2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. |
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The purity of Biotinylated Human TROP-2 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human TROP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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