Biotinylated Human VEGF-C/Flt4-L Protein (VEG-HM4F1B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr103-Arg227

Catalog: VEG-HM4F1B

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Product Description Recombinant Biotinylated Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr103-Arg227.

Product Background

 the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.

Product Category

 Recombinant Protein

Target

 VEGF-C/Flt4-L
Synonyms VEGFC; VEGFc; Flt4-L; VRP; VEGF-C

Gene/Protein ID

 Q6FH59

Species

 Human

Exact Sequence

 Thr103-Arg227
Molecular Weight The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
Biotinylated Human VEGF-C on Tris-Bis PAGE under reduced (R) condition. The purity is greater than 95%.

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