Biotinylated Human VEGF R3/FLT4 Protein (VGF-HM4R3B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human VEGF-C, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr25-Ile776

Catalog: VGF-HM4R3B

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Product Description Recombinant Biotinylated Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776.

Product Background

 Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.

Product Category

 Recombinant Protein

Target

 VEGF R3/FLT4

Gene/Protein ID

 P35916-1

Species

 Human

Exact Sequence

 Tyr25-Ile776
Molecular Weight The protein has a predicted MW of 87.4 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human VEGF-C, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF-C, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 23.0ng/ml determined by ELISA.
The purity of Biotinylated Human VEGF R3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human VEGF R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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