Biotinylated Mouse BCMA/TNFRSF17 Protein (BCM-MM417B)
Catalog: BCM-MM417B
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| Product Description | Recombinant Biotinylated Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Thr49. |
Product Background |
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF). |
Product Category |
Recombinant Protein |
Target |
BCMA/TNFRSF17 |
| Synonyms | CD269; TNFRSF17; BCMA; BCM; TNFRSF13A |
Gene/Protein ID |
O88472-1 |
Species |
Mouse |
Exact Sequence |
Met1-Thr49 |
| Molecular Weight | The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). The affinity constant of 5.64 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse BCMA, His-Avi Tag with an affinity constant of 5.64 uM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). |
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The purity of Biotinylated Mouse BCMA is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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