Biotinylated Mouse IFN alpha/beta R2 Protein (IFN-MM1R2B)
Catalog: IFN-MM1R2B
Order now get it on
| Product Description | Recombinant Biotinylated Mouse IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser22-Ala242. |
Product Background |
Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well. |
Product Category |
Recombinant Protein |
Target |
IFN alpha/beta R2 |
Gene/Protein ID |
O35664-1 |
Species |
Mouse |
Exact Sequence |
Ser22-Ala242 |
| Molecular Weight | The protein has a predicted MW of 28.34 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Biotinylated Mouse IFN alpha/beta R2, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Mouse IFN alpha 1, hFc Tag with an affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). |
|
|
The purity of Biotinylated Mouse IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC. |
|
|
Biotinylated Mouse IFN alpha/beta R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
You may also be interested in
View more associated products