Biotinylated Mouse IL-2 Protein (IL2-MM401B)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Biotinylated: yes
Activity: Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala21-Gln169

Catalog: IL2-MM401B

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Product Description	Recombinant Biotinylated Mouse IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala21-Gln169.
Product Background	Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Product Category	Recombinant Protein

Target	IL-2
Synonyms	IL2; T-cell growth factor; TCGF; Aldesleukin
Gene/Protein ID	P04351-1
Species	Mouse
Exact Sequence	Ala21-Gln169
Molecular Weight	The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA.
	Biotinylated Mouse IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.