Biotinylated Mouse OX40/TNFRSF4/CD134 Protein (OX4-MM440B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Mouse OX40 Ligand, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 4.3ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val20-Pro211

Catalog: OX4-MM440B

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Product Description Recombinant Biotinylated Mouse OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val20-Pro211.

Product Background

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

Product Category

Recombinant Protein

Target

OX40/TNFRSF4/CD134
Synonyms CD134 antigen; CD134;OX40; OX40L receptor; TNFRSF4;ACT-135; ACT35 antigen; ACT35ATC35 antigen; Ly-70; OX40 cell surface antigen; OX40 homologue;- OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen

Gene/Protein ID

P47741

Species

Mouse

Exact Sequence

Val20-Pro211
Molecular Weight The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse OX40 Ligand, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 4.3ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse OX40 Ligand, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 4.3ug/ml determined by ELISA.
The purity of Biotinylated Mouse OX40 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse OX40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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