Biotinylated Mouse PD-1/PDCD1 Protein (Primary Amine Labeling) (PD1-MM201B)
Catalog: PD1-MM201B
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| Product Description | Recombinant Biotinylated Mouse PD-1/PDCD1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Gln167. |
Product Background |
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. |
Product Category |
Recombinant Protein |
Target |
PD-1/PDCD1 |
Gene/Protein ID |
NP_032824 |
Species |
Mouse |
Exact Sequence |
Leu25-Gln167 |
| Molecular Weight | The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 3.35 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Biotinylated Mouse PD-1, hFc Tag with an affinity constant of 3.35 uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Mouse PD-1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Mouse PD-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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