Biotinylated Mouse PLAU/uPA Protein (PLA-MM401B)
Catalog: PLA-MM401B
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| Product Description | Recombinant Biotinylated Mouse PLAU/uPA Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.It contains Gly21-Phe433. |
Product Background |
Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC. |
Product Category |
Recombinant Protein |
Target |
PLAU/uPA |
| Synonyms | PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD |
Gene/Protein ID |
P06869 |
Species |
Mouse |
Exact Sequence |
Gly21-Phe433 |
| Molecular Weight | The protein has a predicted MW of 49.00 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 0.19 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse PLAU, His-Avi Tag with an affinity constant of 0.19 uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Mouse PLAU is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Mouse PLAU on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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