Biotinylated Mouse TNFSF15 Protein (FSF-MM415B)
Catalog: FSF-MM415B
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| Product Description | Recombinant Biotinylated Mouse TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala61-Leu252. |
Product Background |
TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation. |
Product Category |
Recombinant Protein |
Target |
TNFSF15 |
Gene/Protein ID |
Q5UBV8 |
Species |
Mouse |
Exact Sequence |
Ala61-Leu252 |
| Molecular Weight | The protein has a predicted MW of 24.40 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-TNFSF15 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 20.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
| Purity | > 90% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-TNFSF15 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 20.5ng/ml determined by ELISA. |
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Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). |
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Biotinylated Mouse TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. |
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