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Non-biotinylated Human BTLA Protein (BTL-HM401) Summary
Product Description |
Recombinant Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150. |
Product Background |
B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Non-biotinylated Human BTLA Protein (BTL-HM401) Specifications/Details
Protein |
BTLA |
Synonyms |
CD272; BTLA; BTLA1; FLJ16065; MGC129743 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys31-Ser150 |
Molecular Weight |
The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BTLA, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200). |
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Immobilized Human BTLA, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). |
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Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. |
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Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-BTLA Antibody, hFc Tag on ProA-Biosensor can bind Human BTLA, His-Avi Tag with an affinity constant of 2.18 nM as determined in BLI assay . |
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The purity of Human BTLA-is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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