Canine CD40/TNFRSF5 Protein (CD4-DM140)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu21-Ala194

Catalog: CD4-DM140

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Product Description	Recombinant Canine CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Ala194.
Product Background	CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Product Category	Recombinant Protein

Target	CD40/TNFRSF5
Gene/Protein ID	Q7YRL5
Species	Canine
Exact Sequence	Glu21-Ala194
Molecular Weight	The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
	The purity of Canine CD40 is greater than 95% as determined by SEC-HPLC.
	Canine CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.