Canine Coagulation Factor III/Tissue Factor Protein (TFR-DM101)
Catalog: TFR-DM101
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| Product Description | Recombinant Canine Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr35-Glu248. |
Product Background |
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer. |
Product Category |
Recombinant Protein |
Target |
Coagulation Factor III/Tissue Factor |
Gene/Protein ID |
Q4W6L5 |
Species |
Canine |
Exact Sequence |
Thr35-Glu248 |
| Molecular Weight | The protein has a predicted MW of 25.41 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Canine Coagulation Factor III is greater than 95% as determined by SEC-HPLC. |
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Canine Coagulation Factor III on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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