Canine EGF-Protein (EGF-DE101)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Asn973-Arg1024

Catalog: EGF-DE101

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Product Description	Recombinant Canine EGF-Protein is expressed from E.coli with His tag at the N-terminus.It contains Asn973-Arg1024.
Product Background	The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Product Category	Recombinant Protein

Target	EGF
Gene/Protein ID	Q9BEA0
Species	Canine
Exact Sequence	Asn973-Arg1024
Molecular Weight	The protein has a predicted MW of 13.61 kDa. The protein migrates to 16-22 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
	Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The EC50-for this effect is typically 0.5 - 3 ng/mL.
	The purity of Canine EGF is greater than 95% as determined by SEC-HPLC.
	Canine EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.